Hege Vangstein Aamot

• Løvestad, Alexander; Stosic, Milan; Costanzi, Jean-Marc; Christiansen, Irene Kraus; Aamot, Hege Vangstein & Ambur, Ole Herman [Vis alle 7 forfattere av denne artikkelen] (2023). TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling. Virology Journal. ISSN 1743-422X. 20, s. 1–11. doi: 10.1186/s12985-023-02002-5. Fulltekst i vitenarkiv
• Berggreen, Hanne; Løvestad, Alexander; Helmersen, Karin; Jørgensen, Silje Bakken & Aamot, Hege Vangstein (2022). Lessons learned: use of WGS in real-time investigation of suspected intrahospital SARS-CoV-2 outbreaks. Journal of Hospital Infection. ISSN 0195-6701. 131, s. 81–88. doi: 10.1016/j.jhin.2022.10.003. Fulltekst i vitenarkiv
• Steinig, Eike; Aglua, Izzard; Duchene, Sebastian; Meehan, Michael T.; Yoannes, Mition & Firth, Cadhla [Vis alle 34 forfattere av denne artikkelen] (2022). Phylodynamic signatures in the emergence of community-associated MRSA. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 119(45). doi: 10.1073/pnas.2204993119.
• Wegner, Fanny; Roloff, Tim; Huber, Michael; Corday, Samuel; Ramette, Alban & Gerth, Yannik [Vis alle 52 forfattere av denne artikkelen] (2021). External quality assessment of SARS-CoV-2-sequencing: An ESGMD-SSM pilot trial across 15 European laboratories. Journal of Clinical Microbiology. ISSN 0095-1137. 60(1), s. 1–9. doi: 10.1128/JCM.01698-21. Fulltekst i vitenarkiv
• Enger, Hege; Larssen, Kjersti Wik; Damås, Elise Størvold; Aamot, Hege Vangstein; Blomfeldt, Anita & Elstrøm, Petter [Vis alle 7 forfattere av denne artikkelen] (2021). A tale of two STs: molecular and clinical epidemiology of MRSA t304 in Norway 2008–2016. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. s. 1–10. doi: 10.1007/s10096-021-04353-9. Vis sammendrag

  The purpose of this study was to investigate the epidemiological, molecular, and clinical characteristics of MRSA t304/ST8 and t304/ST6 in Norway from 2008 to 2016. Clinical and epidemiological data were collected for each case included in the study. Strains were characterized by PCR, spa typing, antimicrobial susceptibility testing, and whole genome sequencing. The overall number of cases of MRSA t304 increased from 27 in 2008 to 203 in 2016. Most MRSA t304/ST8 cases were defined as HA-MRSA (89.9%) and diagnosed in persons with Norwegian background, many of them living in nursing homes (62.3%). The number of t304/ST8 cases declined throughout the study period and it has not been reported in Norway since 2014. The increasing MRSA t304/ST6 genotype has mainly been introduced to Norway by immigration from the Middle East, but also from other parts of the world. The t304/ST6 clone is mostly classified as CA-MRSA (75.1%), does not seem to cause serious infections, is not multi-resistant, and has not yet caused outbreaks in Norway. This study provides an example of two MRSA clones with the same spa type found in different epidemiological settings. This is very unusual, but still a reminder that spa typing in some cases may have insufficient discriminatory power for surveillance of MRSA. Our results highlight the importance of active surveillance and characterization of emerging MRSA clones with high potential for spread in the community, which may potentially cause outbreaks in healthcare facilities.

• Løvestad, Alexander; Jørgensen, Silje Bakken; Handal, Nina; Ambur, Ole Herman & Aamot, Hege Vangstein (2021). Investigation of intra-hospital SARS-CoV-2 transmission using nanopore whole-genome sequencing. Journal of Hospital Infection. ISSN 0195-6701. 111, s. 107–116. doi: 10.1016/j.jhin.2021.02.022.
• Ferreira, Fabienne Antunes; Helmersen, Karin; Visnovska, Martina; Jørgensen, Silje Bakken & Aamot, Hege Vangstein (2021). Rapid nanopore-based dna sequencing protocol of antibiotic-resistant bacteria for use in surveillance and outbreak investigation. Microbial Genomics. ISSN 2057-5858. 7(4), s. 1–12. doi: 10.1099/MGEN.0.000557. Fulltekst i vitenarkiv
• Noone, John Christopher; Helmersen, Karin; Leegaard, Truls Michael; Skråmm, Inge & Aamot, Hege Vangstein (2021). Rapid Diagnostics of Orthopaedic-Implant-Associated Infections Using Nanopore Shotgun Metagenomic Sequencing on Tissue Biopsies . Microorganisms. ISSN 2076-2607. 9(1). doi: 10.3390/microorganisms9010097. Fulltekst i vitenarkiv
• Michalik, Stefan; Sundaramoorthy, Nandakumar; Murr, Annette; Depke, Maren; Volker, Uwe & Bröker, Barbara M [Vis alle 8 forfattere av denne artikkelen] (2020). Early-Stage Staphylococcus aureus Bloodstream Infection Causes Changes in the Concentrations of Lipoproteins and Acute-Phase Proteins and Is Associated with Low Antibody Titers against Bacterial Virulence Factors. mSystems. ISSN 2379-5077. doi: 10.1128/mSystems.00632-19.
• Blomfeldt, Anita; Jørgensen, Silje Bakken; Helmersen, Karin; Eskonsipo, Petri Kaarlo Juhani & Aamot, Hege Vangstein (2020). Is increased Staphylococcus aureus colonisation during military service caused by specific genotypes? Molecular examination of long-term carriage in a prospective cohort study. APMIS - Journal of Pathologiy, Microbiology and Immunology. ISSN 0903-4641. doi: 10.1111/apm.13106.
• Aamot, Hege Vangstein; Noone, John Christopher; Skråmm, Inge & Leegaard, Truls Michael (2020). Are conventional microbiological diagnostics sufficiently expedient in the era of rapid diagnostics? Evaluation of conventional microbiological diagnostics of orthopedic implant-associated infections (OIAI). Acta Orthopaedica. ISSN 1745-3674. 91, s. 1–4. doi: 10.1080/17453674.2020.1844499. Fulltekst i vitenarkiv
• Noone, John Christopher; Stegger, Marc; Lilje, Berit; Stavem, Knut; Helmersen, Karin & Skråmm, Inge [Vis alle 7 forfattere av denne artikkelen] (2020). Molecular characteristics of Staphylococcus aureus associated prosthetic joint infections after hip fractures treated with hemiarthroplasty: a retrospective genome-wide association study. Scientific Reports. ISSN 2045-2322. 10:16553, s. 1–8. doi: 10.1038/s41598-020-73736-3. Fulltekst i vitenarkiv
• Helmersen, Karin & Aamot, Hege Vangstein (2020). DNA extraction of microbial DNA directly from infected tissue: an optimized protocol for use in nanopore sequencing. Scientific Reports. ISSN 2045-2322. 10(1). doi: 10.1038/s41598-020-59957-6. Fulltekst i vitenarkiv
• Steinig, Elke Joakim; Duchêne, Sebastián; Robinson, D Ashley; Monecke, Stefan; Yokohama, Maho & Laabei, Maisem [Vis alle 38 forfattere av denne artikkelen] (2019). Evolution and global transmission of a multidrug-resistant, community-associated methicillin-resistant staphylococcus aureus lineage from the Indian subcontinent. mBio. ISSN 2161-2129. 10:e01105-19(6), s. 1–20. doi: 10.1128/mBio.01105-19.
• Aamot, Hege Vangstein; Johnsen, Bjørn Odd & Skråmm, Inge (2019). Rapid diagnostics of orthopedic implant-associated infections using Unyvero ITI implant and tissue infection application is not optimal for Staphylococcus species identification. BMC Research Notes. ISSN 1756-0500. 12. doi: 10.1186/s13104-019-4755-5. Vis sammendrag

  Abstract Objectives: This pilot study aimed to compare the commercial Unyvero ITI multiplex PCR application (U-ITI, Curetis GmbH) with conventional culturing concerning (a) detection of pathogens, (b) time to detection of pathogens and (c) time to and quality of antibiotic treatment recommendation in diagnostics of orthopedic implant-associated infections (OIAI). Results: 72 tissue biopsies from 15 consecutive patients with deep OIAI infections were analyzed with conventional culturing including phenotypic antibiotic susceptibility testing and the U-ITI. U-ITI showed lower sensitivity than conventional culturing concerning detection of pathogens (73% vs 93%). 4/15 patients would have been given false negative results by U-ITI, all of which were culture-positive for Staphylococcus species. Median time to detection of pathogens was 47 h and antibiotic resistance 89 h by conventional methods compared to 13.5 h with the U-ITI. The U-ITI did not detect antibiotic resistance, whereas conventional culturing showed resistance to antibiotics covered by the U-ITI panel in 2 patients. Time to detection of pathogens was improved, but the detection limit for staphylococci was unsatisfactory. Although the time to antibiotic treatment recommendation was significantly reduced, the U-ITI would have resulted in incorrect antibiotic recommendation in 2 patients. Our data do not support use of this assay in diagnostics. Keywords: Rapid diagnostics, Implant infection, Staphylococcus aureus, Unyvero ITI, Orthopedic

• Aamot, Hege Vangstein; Eskonsipo, Petri; Jørgensen, Silje Bakken & Blomfeldt, Anita (2018). Staphylococcus aureus colonization during military service: a prospective cohort study. Clinical Microbiology and Infection (CMI). ISSN 1198-743X. 24(7), s. 744–748. doi: 10.1016/j.cmi.2017.10.012.
• Fladberg, Øyvind Andreas; Jørgensen, Silje Bakken & Aamot, Hege Vangstein (2017). Genotypic characterization of gentamicin and cephalosporin 1 resistant Escherichia coli 2 isolates from blood cultures in a Norwegian university hospital 2011-2015. Antimicrobial Resistance and Infection Control. ISSN 2047-2994. 6(1), s. 1–7. doi: 10.1186/s13756-017-0280-2.
• Smeland, Tom Eirik; Müller, Fredrik; Blomfeldt, Anita; Stavem, Knut & Aamot, Hege Vangstein (2017). No associations established between single nucleotide polymorphisms in human Toll-like receptor 2 and Toll-interacting protein and Staphylococcus aureus bloodstream infections. APMIS - Journal of Pathologiy, Microbiology and Immunology. ISSN 0903-4641. 125(10), s. 927–932. doi: 10.1111/apm.12734.
• Blomfeldt, Anita; Larssen, Kjersti Wik; Moghen, Arsalan; Gabrielsen, Christina; Elstrøm, Petter & Aamot, Hege Vangstein [Vis alle 7 forfattere av denne artikkelen] (2017). Emerging multidrug-resistant Bengal Bay clone ST772-MRSA-V in Norway: molecular epidemiology 2004-2014. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. 36(10), s. 1911–1921. doi: 10.1007/s10096-017-3014-8.
• Blomfeldt, Anita; Larssen, Kjersti Wik; Moghen, Arsalan; Haugum, Kjersti; Steen, Tore Wælgaard & Jørgensen, Silje Bakken [Vis alle 7 forfattere av denne artikkelen] (2017). Bengal Bay clone ST772-MRSA-V outbreak: conserved clone causes investigation challenges. Journal of Hospital Infection. ISSN 0195-6701. 95(3), s. 253–258. doi: 10.1016/j.jhin.2016.12.006.
• Blomfeldt, Anita; Eskesen, Arne N; Aamot, Hege Vangstein; Leegaard, Truls Michael & Bjørnholt, Jørgen (2016). Population-based epidemiology of Staphylococcus aureus bloodstream infection: clonal complex 30 genotype is associated with mortality. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. 35(5), s. 803–813. doi: 10.1007/s10096-016-2601-4.
• Blomfeldt, Anita; Aamot, Hege Vangstein; Eskesen, Arne N; Monecke, Stefan; White, Richard & Leegaard, Truls Michael [Vis alle 7 forfattere av denne artikkelen] (2016). DNA microarray analysis of Staphylococcus aureus causing bloodstream infection: bacterial genes associated with mortality? European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. 35(8), s. 1285–1295. doi: 10.1007/s10096-016-2663-3.
• Blomfeldt, Anita; Hasan, Abdullahi Abdi & Aamot, Hege Vangstein (2016). Can MLVA differentiate among endemic-like MRSA isolates with identical spa-type in a low-prevalence region? PLOS ONE. ISSN 1932-6203. 11(2). doi: 10.1371/journal.pone.0148772. Fulltekst i vitenarkiv Vis sammendrag

  The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Norway is low, but an endemic-like MRSA clone with Staphylococcal protein A (spa)-type t304 has been established especially in nursing homes in the Oslo region causing several large outbreaks. The challenge was that spa-typing and the gold standard Pulsed-Field Gel Electrophoresis (PFGE) were inadequate in discriminating isolates in outbreak investigations. Additional higher resolution genotyping methods were needed. The aims of this study were a) to evaluate whether Multiple-Locus Variable number of tandem repeat Analysis (MLVA) could differentiate within the PFGE clusters between epidemiologically related and unrelated endemic-like ST8-MRSA-IV-t304-PVL-neg (MRSA-t304) isolates and b) investigate the evolution of the endemic-like MRSA-t304 clone over a 15-year time period. All MRSA-t304 isolates detected in the region from 1998 through April 2013 were included. In total, 194 of 197 isolates were available for PFGE and MLVA analyses. PFGE results on isolates from 1998–2010 have been published previously. Two PFGE clusters subdivided into eight MLVA types were detected. One major outbreak clone (PFGE cluster C2/ MLVA type MT5045) appeared from 2004 to 2011 causing long-lasting and large outbreaks in seven nursing homes and one hospital. Five new MLVA types (N = 9 isolates) differing in only one VNTR compared to the outbreak clone C2/MT5045 were detected, but only one (C2/MT5044) was seen after 2011. We suggest that MLVA can replace PFGE analysis, but MLVA may not be the optimal method in this setting as it did not discriminate between all epidemiologically unrelated isolates. The results may indicate that all eight outbreaks in different locations within the PFGE C2 cluster may be branches of one large regional outbreak. The major outbreak strain C2/MT5045 may now, however, be under control, extinguished or has moved geographically.

• Aamot, Hege Vangstein; Stavem, Knut & Skråmm, Inge (2015). No change in the distribution of types and antibiotic resistance in clinical Staphylococcus aureus isolates from orthopaedic patients during a period of 12 years. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. 34(9), s. 1833–1837. doi: 10.1007/s10096-015-2420-z.
• Stefan, Monecke; Aamot, Hege Vangstein; Bettina, Stieber; Antje, Ruppelt & Ralf, Ehricht (2014). Characterization of PVL-positive MRSA from Norway. APMIS - Journal of Pathologiy, Microbiology and Immunology. ISSN 0903-4641. 122(7), s. 580–584. doi: 10.1111/apm.12181.
• Fiskvik, Idun Hege; Aamot, Hege Vangstein; Delabie, Jan; Smeland, Erlend B; Stokke, Trond & Heim, Sverre [Vis alle 7 forfattere av denne artikkelen] (2013). Karyotyping of diffuse large B-cell lymphomas: loss of 17p is associated with poor patient outcome. European Journal of Haematology. ISSN 0902-4441. 91(4), s. 332–338. doi: 10.1111/ejh.12171.
• Blomfeldt, Anita; Aamot, Hege Vangstein; Eskesen, Arne N; Müller, Fredrik & Monecke, Stefan (2013). Molecular Characterization of Methicillin-Sensitive Staphylococcus aureus Isolates from Bacteremic Patients in a Norwegian University Hospital. Journal of Clinical Microbiology. ISSN 0095-1137. 51(1), s. 345–347. doi: 10.1128/JCM.02571-12.
• Aamot, Hege Vangstein; Blomfeldt, Anita; Skråmm, Inge; Müller, Fredrik & Monecke, S. (2012). Molecular characterisation of methicillin-sensitive Staphylococcus aureus from deep surgical site infections in orthopaedic patients. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. 31(8), s. 1999–2004. doi: 10.1007/s10096-011-1532-3.
• Aamot, Hege Vangstein; Blomfeldt, Anita & Eskesen, Arne N (2012). Genotyping of 353 Staphylococcus aureus Bloodstream Isolates Collected between 2004 and 2009 at a Norwegian University Hospital and Potential Associations with Clinical Parameters. Journal of Clinical Microbiology. ISSN 0095-1137. 50(9), s. 3111–3114. doi: 10.1128/JCM.01352-12.
• Thorsen, Jim; Aamot, Hege Vangstein; Roberto, Roberta; Tjønnfjord, Geir Erland; Micci, Francesca & Heim, Sverre (2012). Myelodysplastic syndrome with a t(2;11)(p21;q23-24) and translocation breakpoint close to miR-125b-1. Cancer Genetics and Cytogenetics. ISSN 0165-4608. 205(10), s. 528–532. doi: 10.1016/j.cancergen.2012.06.003.
• Eide, Marianne Brodtkorb; Liestøl, Knut; Lingjærde, Ole Christian; Hystad, Marit Elise; Kresse, Stine Henrichson & Meza, Leonardo Zepeda [Vis alle 12 forfattere av denne artikkelen] (2010). Genomic alterations reveal potential for higher grade transformation in follicular lymphoma and confirm parallel evolution of tumor cell clones. Blood. ISSN 0006-4971. 116(9), s. 1489–1497. doi: 10.1182/blood-2010-03-272278.
• Ikonomou, IM; Aamot, Hege; Heim, Sverre; Fosså, Alexander & Delabie, Jan (2007). Granulomatous slack skin with a translocation. American Journal of Surgical Pathology. ISSN 0147-5185. 31(5), s. 803–806.
• Aamot, Hege; Torlakovic, Emina; Eide, Marianne Brodtkorb; Holte, Harald & Heim, Sverre (2007). Non-Hodgkin lymphoma with t(14;18): clonal evolution patterns and cytogenetic-pathologic-clinical correlations. Journal of Cancer Research and Clinical Oncology. ISSN 0171-5216. 133(7), s. 455–470. doi: 10.1007/s00432-006-0188-3.
• Aamot, Hege; Tjønnfjord, Geir Erland; Delabie, Jan & Heim, Sverre (2006). Molecular cytogenetic analysis of leukemic mantle cell lymphoma with a cryptic t(11;14). Cancer Genetics and Cytogenetics. ISSN 0165-4608. 165.
• Ikonomou, IM; Tierens, Anne; Troen, G; Aamot, Hege Vangstein; Heim, Sverre & Lauritzsen, Grete Fossum [Vis alle 8 forfattere av denne artikkelen] (2006). Peripheral T-cell lymphoma with involvement of the expanded mantle zone. Virchows Archiv. ISSN 0945-6317. 449, s. 78–87.
• Emina E, Torlakovic; Aamot, Hege Vangstein & Heim, Sverre (2006). A marginal zone phenotype in follicular lymphoma with t(14;18) is associated with secondary cytogenetic aberrations typical of marginal zone lymphoma. Journal of Pathology. ISSN 0022-3417. 209(2), s. 258–264.
• Aamot, Hege; Bjørnslett, Merete; Delabie, Jan & Heim, Sverre (2005). t(14;22)(q32:q11) in non-Hodgkin lymphoma and myeloid leukaemia: molecular cytogenetic investigations. British Journal of Haematology. ISSN 0007-1048. 130, s. 845–851. doi: 10.1111/j.1365-2141.2005.05688.

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• Jomaas, Ingvild; Helmersen, Karin; Aamot, Hege Vangstein & Blomfeldt, Anita (2023). Can nanopore metagenomic next-generation sequencing replace 16S rRNA Sanger sequencing and give same-day diagnostics?
• Aamot, Hege Vangstein (2023). Molecular diagnostics – what, when and how.
• Aamot, Hege Vangstein (2023). Molecular epidemiology of infectious diseases.
• Aamot, Hege Vangstein (2023). Applying metagenomics in diagnostics – The „technology expert‘s“ perspective.
• Aamot, Hege Vangstein; Claas, Eric C.J.; Couto, Natacha; Westh, Henrik; Greub, Gilbert & Rossen, John W.A. (2022). Next-generation sequencing in routine clinical microbiology and infectious diseases: an ESGMD-ESGEM ESCMID postgraduate course. New microbes and new infections. ISSN 2052-2975. 49-50. doi: 10.1016/j.nmni.2022.101046.
• Noone, John Christopher; Ferreira, Fabienne Antunes & Aamot, Hege Vangstein (2021). Culture-independent genotyping, virulence and antimicrobial resistance gene identification of staphylococcus aureus from orthopaedic implant-associated infections. Microorganisms. ISSN 2076-2607. 9(4). doi: 10.3390/microorganisms9040707. Fulltekst i vitenarkiv Vis sammendrag

  Our culture-independent nanopore shotgun metagenomic sequencing protocol on biopsies has the potential for same-day diagnostics of orthopaedic implant-associated infections (OIAI). As OIAI are frequently caused by Staphylococcus aureus, we included S. aureus genotyping and virulence gene detection to exploit the protocol to its fullest. The aim was to evaluate S. aureus genotyping, virulence and antimicrobial resistance genes detection using the shotgun metagenomic sequencing protocol. This proof of concept study included six patients with S. aureus-associated OIAI at Akershus University Hospital, Norway. Five tissue biopsies from each patient were divided in two: (1) conventional microbiological diagnostics and genotyping, and whole genome sequencing (WGS) of S. aureus isolates; (2) shotgun metagenomic sequencing of DNA from the biopsies. Consensus sequences were analysed using spaTyper, MLST, VirulenceFinder, and ResFinder from the Center for Genomic Epidemiology (CGE). MLST was also compared using krocus. All spa-types, one CGE and four krocus MLST results matched Sanger sequencing results. Virulence gene detection matched between WGS and shotgun metagenomic sequencing. ResFinder results corresponded to resistance phenotype. S. aureus spa-typing, and identification of virulence and antimicrobial resistance genes are possible using our shotgun metagenomics protocol. MLST requires further optimization. The protocol has potential application to other species and infection types.

• Løvestad, Alexander; Handal, Nina; Jørgensen, Silje Bakken; Ambur, Ole Herman & Aamot, Hege Vangstein (2020). OUTBREAK INVESTIGATION OF SARS-COV-2 AMONG HOSPITAL HEALTHCARE WORKERS USING NANOPORE WHOLE-GENOME SEQUENCING.
• Blomfeldt, Anita; Moghen, Arsalan; Aamot, Hege Vangstein; Haugum, Kjersti & Jørgensen, Silje (2016). Emerging multi-resistant Bengal Bay clone ST772-MRSA-V in Norway – cross contamination or parallel import? A cross sectional study.
• Aamot, Hege & Heim, Sverre (2006). Molecular cytogenetic analysis of primary and secondary chromosome abnormalities in B-cell non-Hodgkin lymphoma. Unipub forlag. ISSN 82-8072-408-7.

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