Nina Marie Pedersen

• Wenzel, Eva; Pedersen, Nina Marie; Elfmark, Liv Anker; Ling, Wang; Kjos, Ingrid & Stang, Espen [Show all 10 contributors for this article] (2024). Intercellular transfer of cancer cell invasiveness via endosome-mediated protease shedding. Nature Communications. ISSN 2041-1723. 15. doi: 10.1038/s41467-024-45558-8.
• Holme, Børge; Bjørnerud, Birgitte; Pedersen, Nina Marie; Rodriguez de la Ballina, Laura; Wesche, Jørgen & Haugsten, Ellen Margrethe (2023). Automated tracking of cell migration in phase contrast images with CellTraxx. Scientific Reports. ISSN 2045-2322. 13(1). doi: 10.1038/s41598-023-50227-9. Full text in Research Archive Show summary

  The ability of cells to move and migrate is required during development, but also in the adult in processes such as wound healing and immune responses. In addition, cancer cells exploit the cells’ ability to migrate and invade to spread into nearby tissue and eventually metastasize. The majority of cancer deaths are caused by metastasis and the process of cell migration is therefore intensively studied. A common way to study cell migration is to observe cells through an optical microscope and record their movements over time. However, segmenting and tracking moving cells in phase contrast time-lapse video sequences is a challenging task. Several tools to track the velocity of migrating cells have been developed. Unfortunately, most of the automated tools are made for fluorescence images even though unlabelled cells are often preferred to avoid phototoxicity. Consequently, researchers are constrained with laborious manual tracking tools using ImageJ or similar software. We have therefore developed a freely available, user-friendly, automated tracking tool called CellTraxx. This software makes it easy to measure the velocity and directness of migrating cells in phase contrast images. Here, we demonstrate that our tool efficiently recognizes and tracks unlabelled cells of different morphologies and sizes (HeLa, RPE1, MDA-MB-231, HT1080, U2OS, PC-3) in several types of cell migration assays (random migration, wound healing and cells embedded in collagen). We also provide a detailed protocol and download instructions for CellTraxx.

• Elfmark, Liv Anker; Wenzel, Eva; Ling, Wang; Pedersen, Nina Marie; Stenmark, Harald Alfred & Raiborg, Camilla (2023). Protrudin-mediated ER-endosome contact sites promote phagocytosis. Cellular and Molecular Life Sciences (CMLS). ISSN 1420-682X. 80(8), p. 1–19. doi: 10.1007/s00018-023-04862-0. Full text in Research Archive
• Pedersen, Nina Marie; Wenzel, Eva; Ling, Wang; Antoine, Sandra; Chavrier, Philippe & Stenmark, Harald Alfred [Show all 7 contributors for this article] (2020). Protrudin-mediated ER–endosome contact sites promote MT1-MMP exocytosis and cell invasion. Journal of Cell Biology. ISSN 0021-9525. 219(8), p. 1–28. doi: 10.1083/jcb.202003063. Full text in Research Archive
• Sneeggen, Marte; Pedersen, Nina Marie; Campsteijn, Coen; Haugsten, Ellen Margrethe; Stenmark, Harald & Schink, Kay Oliver (2019). WDFY2 restrains matrix metalloproteinase secretion and cell invasion by controlling VAMP3-dependent recycling. Nature Communications. ISSN 2041-1723. 10(1), p. 1–20. doi: 10.1038/s41467-019-10794-w. Full text in Research Archive
• Wenzel, Eva; Schultz, Sebastian; Schink, Kay Oliver; Pedersen, Nina Marie; Nahse-Kumpf, Viola & Carlson, Andreas [Show all 9 contributors for this article] (2018). Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation. Nature Communications. ISSN 2041-1723. 9(2932), p. 1–18. doi: 10.1038/s41467-018-05345-8. Full text in Research Archive
• Hong, Zhi; Pedersen, Nina Marie; Ling, Wang; Torgersen, Maria Lyngaas; Stenmark, Harald Alfred & Raiborg, Camilla (2017). PtdIns3P controls mTORC1 signaling through lysosomal positioning. Journal of Cell Biology. ISSN 0021-9525. 216(12), p. 4217–4233. doi: 10.1083/jcb.201611073.
• Thorvaldsen, Tor Espen; Pedersen, Nina Marie; Wenzel, Eva & Stenmark, Harald Alfred (2017). Differential Roles of AXIN1 and AXIN2 in Tankyrase Inhibitor-Induced Formation of Degradasomes and β-Catenin Degradation. PLOS ONE. ISSN 1932-6203. 12(1). doi: 10.1371/journal.pone.0170508.
• Raiborg, Camilla; Wenzel, Eva; Pedersen, Nina Marie & Stenmark, Harald Alfred (2016). Phosphoinositides in membrane contact sites. Biochemical Society Transactions. ISSN 0300-5127. 44(2), p. 425–430. doi: 10.1042/BST20150190.
• Raiborg, Camilla; Wenzel, Eva; Pedersen, Nina Marie & Stenmark, Harald Alfred (2016). ER-endosome contact sites in endosome positioning and protrusion outgrowth. Biochemical Society Transactions. ISSN 0300-5127. 44(2), p. 441–446. doi: 10.1042/BST20150246.
• Pedersen, Nina Marie; Thorvaldsen, Tor Espen; Schultz, Sebastian; Wenzel, Eva & Stenmark, Harald Alfred (2016). Formation of tankyrase inhibitor-induced degradasomes requires proteasome activity. PLOS ONE. ISSN 1932-6203. 11(8). doi: 10.1371/journal.pone.0160507.
• Thorvaldsen, Tor Espen; Pedersen, Nina Marie; Wenzel, Eva; Schultz, Sebastian; Brech, Andreas & Liestøl, Knut [Show all 9 contributors for this article] (2015). Structure, dynamics, and functionality of tankyrase inhibitor-induced degradasomes. Molecular Cancer Research. ISSN 1541-7786. 13(11), p. 1487–1501. doi: 10.1158/1541-7786.MCR-15-0125.
• Raiborg, Camilla; Wenzel, Eva; Pedersen, Nina Marie; Olsvik, Hallvard Lauritz; Schink, Kay Oliver & Schultz, Sebastian [Show all 12 contributors for this article] (2015). Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature. ISSN 0028-0836. 520(7546), p. 234–238. doi: 10.1038/nature14359.
• Kostaras, Eleftherios; Pedersen, Nina Marie; Stenmark, Harald; Fotsis, Theodore & Murphy, Carol (2014). SARA and RNF11 at the crossroads of EGFR signaling and trafficking. Methods in Enzymology. ISSN 0076-6879. 535, p. 225–247. doi: 10.1016/B978-0-12-397925-4.00014-6.
• Kostaras, E.; Sflomos, G.; Pedersen, Nina Marie; Stenmark, Harald Alfred; Fotsis, T. & Murphy, C (2013). SARA and RNF11 interact with each other and ESCRT-0 core proteins and regulate degradative EGFR trafficking. Oncogene. ISSN 0950-9232. 32(44), p. 5220–5232. doi: 10.1038/onc.2012.554.
• Oppelt, Angela; Lobert, Viola Helene; Haglund, Kaisa; Mackey, Ashley M.; Rameh, Lucia E. & Liestøl, Knut [Show all 14 contributors for this article] (2013). Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. EMBO Reports. ISSN 1469-221X. 14(1), p. 57–64. doi: 10.1038/embor.2012.183.
• Pedersen, Nina Marie; Raiborg, Camilla; Brech, Andreas; Skarpen, Ellen; Roxrud, Ingrid & Platta, Harald W. [Show all 8 contributors for this article] (2012). The PtdIns3P-Binding Protein Phafin 2 Mediates Epidermal Growth Factor Receptor Degradation by Promoting Endosome Fusion. Traffic: The moving front of cell biology. ISSN 1398-9219. 13(11), p. 1547–1563. doi: 10.1111/j.1600-0854.2012.01400.x.
• Oksvold, Morten Pedersen; Pedersen, Nina Marie; Forfang, Lise & Smeland, Erlend B (2012). Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling. FEBS Letters. ISSN 0014-5793. 586(20), p. 3575–3581. doi: 10.1016/j.febslet.2012.08.022.
• Sak, Malgorzata Magdalena; Breen, Kamilla; Rønning, Sissel Beate; Pedersen, Nina Marie; Bertelsen, Vibeke & Stang, Espen [Show all 7 contributors for this article] (2012). The oncoprotein ErbB3 is endocytosed in the absence of added ligand in a clathrin-dependent manner. Carcinogenesis. ISSN 0143-3334. 33(5), p. 1031–1039. doi: 10.1093/carcin/bgs128.
• Waaler, Jo; Machon, Ondrej; Tumova, Lucie; Dinh, Huyen Mong Thi; Korinek, Vladimir & Wilson, Steven Ray Haakon [Show all 14 contributors for this article] (2012). A novel tankyrase Inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice. Cancer Research. ISSN 0008-5472. 72(11), p. 2822–2832. doi: 10.1158/0008-5472.CAN-11-3336.
• Rønning, Sissel Beate; Pedersen, Nina Marie; Madshus, Inger Helene & Stang, Espen (2011). CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor. Experimental Cell Research. ISSN 0014-4827. 317(13), p. 1804–1816. doi: 10.1016/j.yexcr.2011.05.016.
• Malerød, Lene; Pedersen, Nina Marie; Wegner, Catherine E Sem; Lobert, Viola Helene; Leithe, Edward & Brech, Andreas [Show all 9 contributors for this article] (2011). Cargo-Dependent Degradation of ESCRT-I as a Feedback Mechanism to Modulate Endosomal Sorting. Traffic: The moving front of cell biology. ISSN 1398-9219. 12(9), p. 1211–1226. doi: 10.1111/j.1600-0854.2011.01220.x.
• Thoresen, Sigrid Bratlie; Pedersen, Nina Marie; Liestøl, Knut & Stenmark, Harald Alfred (2010). A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic. Experimental Cell Research. ISSN 0014-4827. 316(20), p. 3368–3378. doi: 10.1016/j.yexcr.2010.07.008.
• Sagona, Antonia; Nezis, Jonnis P; Pedersen, Nina Marie; Liestøl, Knut; Poulton, J & Rusten, Tor Erik [Show all 9 contributors for this article] (2010). PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody. Nature Cell Biology. ISSN 1465-7392. 12(4), p. 362–U140. doi: 10.1038/ncb2036.
• Lobert, Viola Helene; Brech, Andreas; Pedersen, Nina Marie; Wesche, Jørgen; Oppelt, Angela & Malerød, Lene [Show all 7 contributors for this article] (2010). Ubiquitination of alpha 5 beta 1 lntegrin Controls Fibroblast Migration through Lysosomal Degradation of Fibronectin-lntegrin Complexes. Developmental Cell. ISSN 1534-5807. 19(1), p. 148–159. doi: 10.1016/j.devcel.2010.06.010.
• Wegner, Catherine E Sem; Malerød, Lene; Pedersen, Nina Marie; Progida, Cinzia Anita Maria; Bakke, Oddmund & Stenmark, Harald Alfred [Show all 7 contributors for this article] (2010). Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5. Histochemistry and Cell Biology. ISSN 0948-6143. 133(1), p. 41–57. doi: 10.1007/s00418-009-0643-8. Show summary

  (Catherine Sem Wegener adresserer med Centre for Cancer Biomedicine, UiO, må meldes inn i SAP.)

• Pedersen, Nina Marie; Breen, Kamilla; Rødland, Marianne Skeie; Haslekås, Camilla; Stang, Espen & Madshus, Inger Helene (2009). Expression of Epidermal Growth Factor Receptor or ErbB3 Facilitates Geldanamycin-Induced Down-Regulation of ErbB2. Molecular Cancer Research. ISSN 1541-7786. 7(2), p. 275–284. doi: 10.1158/1541-7786.MCR-07-2183.
• Roxrud, Ingrid; Raiborg, Camilla; Pedersen, Nina Marie; Stang, Espen & Stenmark, Harald Alfred (2008). An endosomally localized isoform of Eps15 interacts with Hrs to mediate degradation of epidermal growth factor receptor. Journal of Cell Biology. ISSN 0021-9525. 180.
• Raiborg, Camilla; Malerød, Lene; Pedersen, Nina Marie & Stenmark, Harald Alfred (2008). Differential functions of Hrs and ESCRT proteins in endocytic membrane trafficking. Experimental Cell Research. ISSN 0014-4827. 314, p. 801–813. doi: 10.1016/j.yexcr.2007.10.014.
• Pedersen, Nina Marie; Madshus, Inger Helene; Haslekås, Camilla & Stang, Espen (2008). Geldanamycin-induced down-regulation of ErbB2 from the plasma membrane is clathrin dependent but proteasomal activity independent. Molecular Cancer Research. ISSN 1541-7786. 6. doi: 10.1158/1541-7786.MCR-07-0191.
• Johannessen, Lene Elisabeth; Pedersen, Nina Marie; Pedersen, Ketil Winther; Madshus, Inger Helene & Stang, Espen (2006). Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits. Molecular and Cellular Biology. ISSN 0270-7306. 26, p. 389–401.
• Kazazic, Maja; Roepstorff, K; Johannessen, Lene Elisabeth; Pedersen, Nina Marie; van Deurs, B & Stang, Espen [Show all 7 contributors for this article] (2006). EGF-induced activation of the EGF receptor does not trigger mobilization of caveolae. Traffic: The moving front of cell biology. ISSN 1398-9219. 7, p. 1518–1527.
• Longva, Karianne Eide; Pedersen, Nina Marie; Haslekås, Camilla; Stang, Espen & Madshus, Inger Helene (2005). Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2. International Journal of Cancer. ISSN 0020-7136. 116, p. 359–367.

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• Pedersen, Nina Marie (2023). Collagen as a tool to study Invasive Cell Migration in a 3D matrix .
• Pedersen, Nina Marie (2023). Tenk som er forsker .
• Pedersen, Nina Marie (2007). Endocytic downregulation of the receptor tyrosine kinases EGFR and ErbB2. Unipub forlag. ISSN 978-82-8072-700-8.

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