Lars Oliver Baumbusch

Norwegian version of this page Department of Nursing, Health and laboratory science

Norwegian version of this page Position

Professor

Contact

lars.o.baumbusch@hiof.no

Study place

Fredrikstad

Office nr.

S428

Academic interests

Molecular biology, "omics", biotechnology, bioethics, applied bioinformatics, oxidative stress, and childhood cancer research.

Courses taught

IRBIO30220 Molekylær diagnostikk og bioinformatikk
IRBIO30120 Innovasjon og drift av medisinske laboratorier
IRBIO30320 Bachelorprosjekt med naturvitenskapelig metode og profesjonspraksis

Background

Lars O. Baumbusch graduated with an MSc (diploma) degree in biology from the Albert-Ludwig University of Freiburg, Germany. He obtained his PhD (Dr. scient.) from the University of Oslo, Norway in 2001. In his thesis, he analyzed genes controlling embryogenesis and embryo dormancy in plants, including chromatin-modulating SET domain genes.

A post-doc career stipend, awarded from the Norwegian Research Council, provided him with the opportunity to study gene regulation and dysregulation in breast tumor development and progression at the Institute for Cancer Research, Oslo University Hospital Radiumhospitalet. He continued his scientific career (as a post-doc, scientist, and project group leader) at the same institution, applying advanced bioinformatic tools for copy number analysis. Moreover, he examined genomic instability in primary breast and ovarian tumors and molecular signatures of circulating or disseminated tumor cells (micrometastasis) in breast cancer. One of his projects, focusing on long non-protein coding RNAs profiles in breast cancer, was honoured with the EACR Presidential Session Research Award at the EACR-21 in 2010.

In addition, he was engaged as an adjunct associate professor at the University of Oslo from 2006 to 2010. During the spring semesters 2018 until 2021, has been employed as an associate professor and full professor at the Oslo Metropolitan University, teaching a Bsc course in molecular biology. Today, he holds an adjunct professor (professor II) position at the Østfold University College, Fredrikstad, Norway.

Since 2012, he is working at the Dep. of Pediatric Research, Oslo University Hospital Rikshospitalet investigating oxidative stress models and novel biomarkers for asphyxia damages. His scientific interests cover also how modern single cell and sequencing technologies can be applied to explore childhood cancers, in particular high-risk neuroblastoma. To this end, he is one of the co-founders of the Norwegian childhood cancer biobank. For more details about the scientific projects, publications, and perspectives of LOB please see https://ous-research.no/baumbusch.

Awards

2010 Presidential Session Research Award, EACR-21, Oslo
2002 Career stipend, Functional Genomic Research in Norway (FUGE) program, Research Council of Norway (NFR)

Appointments

2019 - present: Norwegian Association of Researchers, board member at OUS

Positions held

04.2021 - present: Senior scientist and group leader at the Dep. of Pediatric Research (PFI), Division of Paediatric and Adolescent Medicine, Oslo University Hospital (OUS) Rikshospitalet, Oslo, Norway
01.-05.2018 - 2021: Professor / associate professor (førsteamanuensis) at the Dep. of Mechanical, Electronics and Chemical Engineering, Oslo Metropolitan University (OsloMet), Oslo, Norway
11.2012 - 03.2021: Scientist at the Dep. of Pediatric Research, Division of Paediatric and Adolescent Medicine, Oslo University Hospital Rikshospitalet, Oslo, Norway

Partners

Oslo University Hospital Rikshospitalet (https://ous-research.no/baumbusch)

de Muinck, Eric; Trosvik, Pål; Nguyen, Nga; Fashing, Peter J.; Stigum, Vetle Malmer & Robinson, Nina [Show all 9 contributors for this article] (2023). Reduced abundance of Faecalibacterium prausnitzii in the gut microbiota of children diagnosed with cancer, a pilot study. Frontiers in Mircobiomes. ISSN 2813-4338. 2. doi: 10.3389/frmbi.2023.1151889. Full text in Research Archive
Tune, Benedicte Grebstad; Sareen, Heena; Powter, Branka; Kahana-Edwin, Smadar; Cooper, Adam & Koh, Eng-Siew [Show all 21 contributors for this article] (2023). From Pediatric to Adult Brain Cancer: Exploring Histone H3 Mutations in Australian Brain Cancer Patients. Biomedicines. 11(11). doi: 10.3390/biomedicines11112907. Full text in Research Archive
Tune, Benedicte Grebstad; Melheim, Maria; Atneosen-Åsegg, Monica; Dotinga, Baukje; Saugstad, Ola Didrik & Solberg, Rønnaug [Show all 7 contributors for this article] (2023). Long Non-Coding RNAs in Hypoxia and Oxidative Stress: Novel Insights Investigating a Piglet Model of Perinatal Asphyxia. Biology (Basel). ISSN 2079-7737. 12(4). doi: 10.3390/biology12040549. Full text in Research Archive
Bitenc, Marie; Tune, Benedicte Grebstad; Melheim, Maria; Atneosen-Åsegg, Monica; Lai, Xiaoran & Rajar, Polona [Show all 8 contributors for this article] (2022). Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia. Molecular Biology Reports. ISSN 0301-4851. 50, p. 1533–1544. doi: 10.1007/s11033-022-08135-0. Full text in Research Archive
Przybyla, Weronika; Paulsen, Kirsti; Mishra, Charitra Kumar; Nygård, Ståle; Engebretsen, Solveig & Ruud, Ellen [Show all 9 contributors for this article] (2022). Whole exome sequencing of high-risk neuroblastoma identifies novel non-synonymous variants. PLOS ONE. ISSN 1932-6203. 17(8), p. 1–24. doi: 10.1371/journal.pone.0273280. Full text in Research Archive Show summary
Neuroblastoma (NBL), one of the main death-causing cancers in children, is known for its remarkable genetic heterogeneity and varied patient outcome spanning from spontaneous regression to widespread disease. Specific copy number variations and single gene rearrangements have been proven to be associated with biological behavior and prognosis; however, there is still an unmet need to enlarge the existing armamentarium of prognostic and therapeutic targets. We performed whole exome sequencing (WES) of samples from 18 primary tumors and six relapse samples originating from 18 NBL patients. Our cohort consists of 16 high-risk, one intermediate, and one very low risk patient. The obtained results confirmed known mutational hotspots in ALK and revealed other non-synonymous variants of NBL-related genes (TP53, DMD, ROS, LMO3, PRUNE2, ERBB3, and PHOX2B) and of genes cardinal for other cancers (KRAS, PIK3CA, and FLT3). Beyond, GOSeq analysis determined genes involved in biological adhesion, neurological cell-cell adhesion, JNK cascade, and immune response of cell surface signaling pathways. We were able to identify novel coding variants present in more than one patient in nine biologically relevant genes for NBL, including TMEM14B, TTN, FLG, RHBG, SHROOM3, UTRN, HLA-DRB1, OR6C68, and XIRP2. Our results may provide novel information about genes and signaling pathways relevant for the pathogenesis and clinical course in high-risk NBL.
Hermansen, Johanne Uthus; Wojcik, Dorota; Robinson, Nina; Pahnke, Jens; Haugland, Hans Kristian & Jamtøy, Ann-Helen [Show all 11 contributors for this article] (2021). The Norwegian childhood cancer biobank. Cancer Reports. ISSN 2573-8348. 0:e1555(8), p. 1–8. doi: 10.1002/cnr2.1555. Full text in Research Archive Show summary
Background The rapidly expanding era of “omics” research is highly dependent on the availability of quality-proven biological material, especially for rare conditions such as pediatric malignancies. Professional biobanks provide such material, focusing on standardized collection and handling procedures, distinctive quality measurements, traceability of storage conditions, and accessibility. For pediatric malignancies, traditional tumor biobanking is challenging due to the rareness and limited amount of tissue and blood samples. The higher molecular heterogeneity, lower mutation rates, and unique genomic landscapes, however, renders biobanking of this tissue even more crucial. Aim The aim of this study was to test and establish methods for a prospective and centralized biobank for infants, children, and adolescents up to 18 years of age diagnosed with cancer in Norway. Methods Obtain judicial and ethical approvals and administration through a consortium, steering committee, and advisory board. Develop pipelines including SOPs for all aspects in the biobank process, including collection, processing and storing of samples and data, as well of quality controlling, safeguarding, distributing, and transport. Results The childhood cancer biobanking started at Oslo University Hospital in March 2017 and was from 2019 run as a national Norwegian Childhood Cancer Biobank. Informed consent and biological samples are collected regionally and stored centrally. Approximately 12 000 samples from 510 patients and have been included by January 1, 2021, representing a 96% consent and participation rate among our newly diagnosed patients. Conclusion A well-functioning nationwide collection and centralized biobank with standardized procedures and national storage for pediatric malignancies has been established with a high acceptance among families.
Zasada, Magdalena; Madetko-Talowska, Anna; Revhaug, Cecilie; Rognlien, Anne Gro Wesenberg; Baumbusch, Lars Oliver & Książek, Teofila [Show all 12 contributors for this article] (2020). Transcriptome analysis reveals dysregulation of genes involved in oxidative phosphorylation in a murine model of retinopathy of prematurity. Pediatric Research. ISSN 0031-3998. 88(3), p. 391–397. doi: 10.1038/s41390-020-0793-x.
Zasada, Magdalena; Suski, Maciej; Bokiniec, Renata; Szwarc-Duma, Monika; Borszewska-Kornacka, Maria Katarzyna & Madej, Józef [Show all 13 contributors for this article] (2019). Comparative two time-point proteome analysis of the plasma from preterm infants with and without bronchopulmonary dysplasia. The Italian Journal of Pediatrics. ISSN 1720-8424. 45(112), p. 1–9. doi: 10.1186/s13052-019-0676-0. Full text in Research Archive
Rajar, Polona; Atneosen-Åsegg, Monica; Saugstad, Ola Didrik; Solberg, Rønnaug & Baumbusch, Lars Oliver (2019). Quantification of circulating cell-free DNA (cfDNA) in urine using a newborn piglet model of asphyxia. PLOS ONE. ISSN 1932-6203. 14 (12), p. 1–14. doi: 10.1371/journal.pone.0227066. Full text in Research Archive
Revhaug, Cecilie; Bik-Multanowski, Miroslaw; Zasada, Magdalena; Rognlien, Anne Gro Wesenberg; Günther, Clara-Cecilie & Ksiazek, Teofila [Show all 13 contributors for this article] (2019). Immune System Regulation Affected by a Murine Experimental Model of Bronchopulmonary Dysplasia: Genomic and Epigenetic Findings. Neonatology. ISSN 1661-7800. 116, p. 269–277. doi: 10.1159/000501461. Full text in Research Archive Show summary
Background: Bronchopulmonary dysplasia (BPD) is a common cause of abrupted lung development after preterm birth. BPD may lead to increased rehospitalization, more severe and frequent respiratory infections, and life-long reduced lung function. The gene regulation in lungs with BPD is complex, with various genetic and epigenetic factors involved. Objectives: The aim of this study was to examine the regulatory relation between gene expression and the epigenome (DNA methylation) relevant for the immune system after hyperoxia followed by a recovery period in air using a mouse model of BPD. Methods: Newborn mice pups were subjected to an immediate hyperoxic condition from birth and kept at 85% O2 levels for 14 days followed by a 14-day period in room air. Next, mice lung tissue was used for RNA and DNA extraction with subsequent microarray-based assessment of lung transcriptome and supplementary methylome analysis. Results:The immune system-related transcriptomeregulation was affected in mouse lungs after hyperoxia. A high proportion of genes relevant in the immune system exhibited significant expression alterations, e.g., B cell-specific genes central to the cytokine-cytokine receptor interaction, the PI3K-AKT, and the B cell receptor signaling pathways. The findings were accompanied by significant DNA hypermethylation observed in the PI3K-AKT pathway and immune system-relevant genes. Conclusions: Oxygen damage could be partly responsible for the increased susceptibility and abnormal response to respiratory viruses and infections seen in premature babies with BPD through dysregulated genes.
Zasada, Magdalena; Madetko-Talowska, Anna; Revhaug, Cecilie; Rognlien, Anne Gro Wesenberg; Baumbusch, Lars Oliver & Książek, Teofila [Show all 12 contributors for this article] (2019). Short- and long-term impact of hyperoxia on the blood and retinal cells' transcriptome in a mouse model of oxygen-induced retinopathy. Pediatric Research. ISSN 0031-3998. 87, p. 485–493. doi: 10.1038/s41390-019-0598-y. Full text in Research Archive
Revhaug, Cecilie; Zasada, Magdalena; Rognlien, Anne Gro Wesenberg; Günther, Clara-Cecilie; Grabowska, Agnieszka & Książek, Teofila [Show all 13 contributors for this article] (2019). Pulmonary vascular disease is evident in gene regulation of experimental bronchopulmonary dysplasia. Journal of Maternal-Fetal & Neonatal Medicine. ISSN 1476-7058. doi: 10.1080/14767058.2018.1541081. Full text in Research Archive Show summary
Objective: To examine the gene expression regarding pulmonary vascular disease in experimental bronchopulmonary dysplasia in young mice. Premature delivery puts babies at risk of severe complications. Bronchopulmonary dysplasia (BPD) is a common complication of premature birth leading to lifelong affection of pulmonary function. BPD is recognized as a disease of arrested alveolar development. The disease process is not fully described and no complete cure or prevention is known. The focus of interest in the search for treatment and prevention of BPD has traditionally been at airspace level; however, the pulmonary vasculature is increasingly acknowledged in the pathology of BPD. The aim of the investigation was to study the gene expression in lungs with BPD with regards to pulmonary vascular disease (PVD). Methods: We employed a murine model of hyperoxia-induced BPD and gene expression microarray technique to determine the mRNA expression in lung tissue from young mice. We combined gene expression pathway analysis and analyzed the biological function of multiple single gene transcripts from lung homogenate to study the PVD relevant gene expression. Results: There were n = 117 significantly differentially regulated genes related to PVD through down-regulation of contractile elements, up- and down-regulation of factors involved in vascular tone and tissue-specific genes. Several genes also allowed for pinpointing gene expression differences to the pulmonary vasculature. The gene Nppa coding for a natriuretic peptide, a potent vasodilator, was significantly down-regulated and there was a significant up-regulation of Pde1a (phosphodiesterase 1A), Ptger3 (prostaglandin e receptor 3), and Ptgs1 (prostaglandin-endoperoxide synthase one). Conclusion: The pulmonary vasculature is affected by the arrest of secondary alveolarization as seen by differentially regulated genes involved in vascular tone and pulmonary vasculature suggesting BPD is not purely an airspace disease. Clues to prevention and treatment may lie in the pulmonary vascular system.
Zasada, Magdalena; Suski, Maciej; Bokiniec, Renata; Szwarc-Duma, Monika; Borszewska-Kornacka, Maria Katarzyna & Madej, Józef [Show all 13 contributors for this article] (2018). An iTRAQ-based quantitative proteomic analysis of plasma proteins in preterm newborns with retinopathy of prematurity. Investigative Ophthalmology and Visual Science. ISSN 0146-0404. 59(13), p. 5312–5319. doi: 10.1167/iovs.18-24914. Full text in Research Archive Show summary
Purpose: Retinopathy of prematurity (ROP) is a vision-threatening complication of a premature birth, in which the etiology still remains unclear. Importantly, the molecular processes that govern these effects can be investigated in a perturbed plasma proteome composition. Thus, plasma proteomics may add new insights into a better understanding of the pathogenesis of this disease. Methods: The cord and peripheral blood of neonates (≤30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (PMA), respectively. Blood samples were retrospectively subdivided into ROP(+) and ROP(−) groups, according to the development of ROP. Results: The quantitative analysis of plasma proteome at both time points revealed 30 protein abundance changes between ROP(+) and ROP(−) groups. After standardization to gestational age, children who developed ROP were characterized by an increased C3 complement component and fibrinogen level at both analyzed time points. Conclusions: Higher levels of the complement C3 component and fibrinogen, present in the cord blood and persistent to 36 PMA, may indicate a chronic low-grade systemic inflammation and hypercoagulable state that may play a role in the development of ROP.
Manueldas, Sophia; Benterud, Torkil; Rueegg, Corina Silvia; Garberg, Håvard Tetlie; Huun, Marianne Ullestad & Pankratov, Leonid [Show all 11 contributors for this article] (2018). Temporal patterns of circulating cell-free DNA (cfDNA) in a newborn piglet model of perinatal asphyxia. PLOS ONE. ISSN 1932-6203. 13:e0206601(11), p. 1–20. doi: 10.1371/journal.pone.0206601. Full text in Research Archive
Suski, Maciej; Bokiniec, Renata; Szwarc-Duma, Monika; Madej, Józef; Bujak-Giżycka, Beata & Kwinta, Przemko [Show all 11 contributors for this article] (2018). Prospective plasma proteome changes in preterm infants with different gestational ages. Pediatric Research. ISSN 0031-3998. 84, p. 104–111. doi: 10.1038/s41390-018-0003-2.
Suski, Maciej; Bokiniec, Renata; Szwarc-Duma, Monika; Madej, Jozef; Bujak-Gizycka, Beata & Borszewska-Kornacka, Maria K. [Show all 13 contributors for this article] (2018). Plasma proteome changes in cord blood samples from preterm infants. Journal of Perinatology. ISSN 0743-8346. 38, p. 1182–1189. doi: 10.1038/s41372-018-0150-7. Full text in Research Archive
Benterud, Torkil; Manueldas, Sophia; Rivera, Santiago; Henckel, Ewa; Løberg, Else Marit & Norgren, Svante [Show all 9 contributors for this article] (2018). Cerebellum Susceptibility to Neonatal Asphyxia: Possible Protective Effects of N-Acetylcysteine Amide. Disease Markers. ISSN 0278-0240. 2018(5046372). doi: 10.1155/2018/5046372. Full text in Research Archive
Kwinta, Przemko; Bokiniec, Renata; Bik-Multanowski, Miroslaw; Günther, Clara-Cecilie; Grabowska, Agnieszka & Ksiazek, Teofila [Show all 14 contributors for this article] (2017). Comparison of whole genome expression profile between preterm and full-term newborns. Ginekologia Polska. ISSN 0017-0011. 88(8), p. 434–441. doi: 10.5603/GP.a2017.0080.
Garberg, Håvard Tetlie; Huun, Marianne Ullestad; Baumbusch, Lars Oliver; Atneosen-Åsegg, Monica; Solberg, Rønnaug & Saugstad, Ola Didrik (2017). Temporal Profile of Circulating microRNAs after Global Hypoxia-Ischemia in Newborn Piglets. Neonatology. ISSN 1661-7800. 111(2), p. 133–139. doi: 10.1159/000449032.
Demeulemeester, Jonas; Kumar, Parveen; Møller, Elen Kristine; Nord, Silje; Wedge, David C. & Peterson, April [Show all 20 contributors for this article] (2016). Tracing the origin of disseminated tumor cells in breast cancer using single-cell sequencing. Genome Biology. ISSN 1465-6906. 17(1). doi: 10.1186/s13059-016-1109-7.
Benterud, Torkil; Ystgaard, Martin Bogale; Manueldas, Sophia; Pankratov, Leonid; Alfaro-Cervello, Clara & Florholmen, Geir [Show all 13 contributors for this article] (2016). N-Acetylcysteine Amide Exerts Possible Neuroprotective Effects in Newborn Pigs after Perinatal Asphyxia. Neonatology. ISSN 1661-7800. 111(1), p. 12–21. doi: 10.1159/000447255.
Kaveh, Fatemeh; Baumbusch, Lars Oliver; Nebdal, Daniel J.H.; Børresen-Dale, Anne-Lise; Lingjærde, Ole Christian & Edvardsen, Hege [Show all 8 contributors for this article] (2016). A systematic comparison of copy number alterations in four types of female cancer. BMC Cancer. ISSN 1471-2407. 16(913). doi: 10.1186/s12885-016-2899-4.
Benterud, Torkil; Pankratov, Leonid; Solberg, Rønnaug; Bolstad, Nils; Skinningsrud, Anders & Baumbusch, Lars Oliver [Show all 8 contributors for this article] (2015). Perinatal asphyxia may influence the level of beta-amyloid (1-42) in cerebrospinal fluid: An experimental study on newborn pigs. PLOS ONE. ISSN 1932-6203. 10(10). doi: 10.1371/journal.pone.0140966.
Gul, Kiran Aftab; Øverland, Torstein; Osnes, Liv T. N.; Baumbusch, Lars Oliver; Pettersen, Folf Dagfinn & Lima, Kari [Show all 7 contributors for this article] (2015). Neonatal Levels of T-cell Receptor Excision Circles (TREC) in Patients with 22q11.2 Deletion Syndrome and Later Disease Features. Journal of Clinical Immunology. ISSN 0271-9142. 35(4), p. 408–415. doi: 10.1007/s10875-015-0153-5.
Reiche, Kristin; Kasack, Katharina; Schreiber, Stephan; Luders, Torben; Due, Eldri Undlien & Naume, Bjørn [Show all 12 contributors for this article] (2014). Long non-coding rnas differentially expressed between normal versus primary breast tumor tissues disclose converse changes to breast cancer-related protein-coding genes. PLOS ONE. ISSN 1932-6203. 9(9). doi: 10.1371/journal.pone.0106076.
Liu, Yanhong; Zhou, R.; Baumbusch, Lars Oliver; Tsavachidis, Spyros; Brewster, Abenaa M. & Do, Kim Anh-Tri [Show all 16 contributors for this article] (2014). Genomic copy number imbalances associated with bone and non-bone metastasis of early-stage breast cancer. Breast Cancer Research and Treatment. ISSN 0167-6806. 143(1), p. 189–201. doi: 10.1007/s10549-013-2796-3.
Baumbusch, Lars Oliver; Helland, Åslaug; Wang, Yun; Liestøl, Knut; Schaner, M.E. & Holm, Ruth [Show all 16 contributors for this article] (2013). High Levels of Genomic Aberrations in Serous Ovarian Cancers Are Associated with Better Survival. PLOS ONE. ISSN 1932-6203. 8(1). doi: 10.1371/journal.pone.0054356.
Aure, Miriam Ragle; Steinfeld, I; Baumbusch, Lars Oliver; Liestøl, Knut; Lipson, Doron & Nyberg, Sandra Johanna [Show all 12 contributors for this article] (2013). Identifying In-Trans Process Associated Genes in Breast Cancer by Integrated Analysis of Copy Number and Expression Data. PLOS ONE. ISSN 1932-6203. 8(1). doi: 10.1371/journal.pone.0053014.
Møller, Elen Kristine; Kumar, P; Voet, Thierry; Peterson, April; Van Loo, Peter & Mathisen, Randi Margit Ruud [Show all 15 contributors for this article] (2013). Next-generation sequencing of disseminated tumor cells. Frontiers of radiation therapy and oncology. ISSN 0071-9676. doi: 10.3389/fonc.2013.00320.
Otto, Benjamin; Gruner, Katharina; Heinlein, Christina; Wegwitz, Florian; Nollau, Peter & Ylstra, Bauke [Show all 15 contributors for this article] (2013). Low-grade and high-grade mammary carcinomas in WAP-T transgenic mice are independent entities distinguished by Met expression. International Journal of Cancer. ISSN 0020-7136. 132(6), p. 1300–1310. doi: 10.1002/ijc.27783.
Ruud Mathiesen, Randi Margit; Fjelldal, Renathe; Liestøl, Knut; Due, Eldri Undlien; Geigl, Jochen & Riethdorf, Sabine [Show all 18 contributors for this article] (2012). High-resolution analyses of copy number changes in disseminated tumor cells of patients with breast cancer. International Journal of Cancer. ISSN 0020-7136. 131(4), p. E405–E415. doi: 10.1002/ijc.26444.
Nilsen, Gro; Liestøl, Knut; Van Loo, P; Vollan, Hans Kristian Moen; Eide, Marianne Brodtkorb & Rueda, Oscar M. [Show all 12 contributors for this article] (2012). Copynumber: Efficient algorithms for single and multi-track copy number segmentation. BMC Genomics. ISSN 1471-2164. 13(591). doi: 10.1186/1471-2164-13-591.
Molloy, Timothy J.; Bosma, Astrid J.; Baumbusch, Lars Oliver; Synnestvedt, Marit; Borgen, Elin & Russnes, Hege Elisabeth Giercksky [Show all 9 contributors for this article] (2011). The prognostic significance of tumour cell detection in the peripheral blood versus the bone marrow in 733 early-stage breast cancer patients. Breast Cancer Research. ISSN 1465-5411. 13(3). doi: 10.1186/bcr2898.
Russnes, Hege Elisabeth Giercksky; Vollan, Hans Kristian Moen; Lingjærde, Ole Christian; Krasnitz, Alexander; Lundin, Pär & Naume, Bjørn [Show all 23 contributors for this article] (2010). Genomic Architecture Characterizes Tumor Progression Paths and Fate in Breast Cancer Patients. Science Translational Medicine. ISSN 1946-6234. 2(38), p. 38–47. doi: 10.1126/scitranslmed.3000611.
Geigl, JB; Obenauf, AC; Waldispuehl-Geigl, J; Hoffmann, EM; Auer, M & Hormann, M [Show all 11 contributors for this article] (2009). Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays. Nucleic Acids Research (NAR). ISSN 0305-1048. 37(15). doi: 10.1093/nar/gkp526.
Baumbusch, Lars Oliver; Aarøe, Jørgen Mømb; Johansen, Finn Erik; Hicks, J; Sun, H & Bruhn, L [Show all 12 contributors for this article] (2008). Comparison of the Agilent, ROMA/NimbleGen and Illumnia platforms for classification of copy numer alterations in human breast tumors. BMC Genomics. ISSN 1471-2164. 9. doi: 10.1186/1471-2164-9-379.
Baumbusch, Lars Oliver; Myhre, Simen; Langerød, Anita; Bergamaschi, Anna; Geisler, Stephanie B & Lønning, Per Eystein [Show all 9 contributors for this article] (2006). Expression of full-length p35 and its isoform DELTAp53 in breast carcinomas in relation to mutation status and clinical parameters. Molecular Cancer. ISSN 1476-4598. 5. doi: 10.1186/1476-4598-5-47.
Lingjærde, Ole Christian; Baumbusch, Lars Oliver; Liestøl, Knut; Glad, Ingrid Kristine & Børresen-Dale, Anne-Lise (2005). CGH-Explorer: a program for analysis of array-CGH data. Bioinformatics. ISSN 1367-4803. 21. doi: 10.1093/bioinformatics/bti113.

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Paulsen, Kirsti; Przybyła, Weronika; Trøen, Gunhild; Beiske, Klaus Hermann & Baumbusch, Lars Oliver (2023). Exploring the heterogeneity of neuroblastoma and other childhood cancers.
Volstad, K; Melheim, Maria; Saugstad, Ola Didrik; Stiris, Tom; Baumbusch, Lars Oliver & Andresen, Jannicke Hanne (2023). Effect of nicotine on brain-derived neurotrophic factor and brain-derived neurotrophic factor antisense in newborn asphyxiated piglets.
Przybyła, Weronika; Trøen, Gunhild; Lai, Xiaoran; Samarakoon, Pubudu Saneth; Kaveh, Fatemeh & Hovig, Eivind [Show all 8 contributors for this article] (2023). Comparative whole genome copy number analysis of disseminated tumor cells and the primary tumor in high-risk neuroblastoma.
Baumbusch, Lars Oliver (2023). Molekylære signaturer i barnekreftsykdommen nevroblastom.
Baumbusch, Lars Oliver (2022). Barnekreft – pågående forskningsprosjekter.
Baumbusch, Lars Oliver (2022). Biobanking, sequencing, and sing cell techniques to explore high-risk neuroblastoma and other childhood cancers.
Baumbusch, Lars Oliver (2022). The Norwegian Childhood Cancer Biobank (NCCB) - experiences and perspectives.
Baumbusch, Lars Oliver (2022). The Norwegian Childhood Cancer Biobank (NCCB) - experiences and challenges.
Kaveh, Fatemeh; Baumbusch, Lars Oliver; Nebdal, Daniel J.H.; Børresen-Dale, Anne-Lise; Lingjærde, Ole Christian & Edvardsen, Hege [Show all 8 contributors for this article] (2018). Correction: A systematic comparison of copy number alterations in four types of female cancer. BMC Cancer. ISSN 1471-2407. 18(80). doi: 10.1186/s12885-017-3766-7.
Baumbusch, Lars Oliver; Helland, Åslaug; Wang, Yun; Liestøl, Knut; Schaner, Marci & Holm, Ruth [Show all 17 contributors for this article] (2012). High levels of genomic aberrations in serous ovarian cancers are associated with better survival.
Baumbusch, Lars Oliver; Reiche, Kristin; Schutt, Katharina; Schreiber, S; Due, Eldri Undlien & Luders, Torben [Show all 10 contributors for this article] (2010). Molecular signatures of long non-coding RNAs in breast cancer patients. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 12–12.
Mathiesen, RR; Fjelldal, R; Due, Eldri U.; Geigl, JB; Riethdorf, S & Børresen-Dale, Anne-Lise [Show all 10 contributors for this article] (2010). Genomic profiles of single tumor cells in metastatic breast cancer patients.
Reiche, Kristin; Baumbusch, Lars Oliver; Schutt, Katharina; Schreiber, Stephan; Due, Eldri U. & Lüders, Torben [Show all 10 contributors for this article] (2010). Molecular signatures of long non-coding RNAs in breast cancer patients.
Baumbusch, Lars Oliver; Reiche, Kristin; Schutt, Katharina; Schreiber, Stephan; Due, Eldri U. & Lüders, Torben [Show all 10 contributors for this article] (2010). Molecular signatures of long non-coding RNAs in breast cancer patients.
Zhao, Xi; Schultz, Nikolaus; Taylor, Barry S.; Cerami, Ethan; Baumbusch, Lars Oliver & Haakensen, Vilde Drageset [Show all 10 contributors for this article] (2010). Pathway signatures in breast cancer progression - a genome-scale study based on integration of biology networks, DNA copy number, gene expression and mutations. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 207–208.
Aarøe, Jørgen Mømb; Myhre, Simen; Vollan, Hans Kristian Moen; Haakensen, Vilde Drageset; Baumbusch, Lars Oliver & Due, Eldri Undlien [Show all 10 contributors for this article] (2010). Measuring the level of genomic distortion in breast tumours with increasing histological grade - a progression model. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 221–221.
Mathiesen, Randi R.; Fjelldal, Renathe; Due, Eldri Undlien; Geigl, Jochen B.; Riethdorf, Sabine & Børresen-Dale, Anne-Lise [Show all 10 contributors for this article] (2010). Genomic profiles of single tumour cells in metastatic breast cancer patients. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 93–94.
Vollan, Hans Kristian Moen; Nilsen, G; Baumbusch, Lars Oliver; Schneider, Ida Johansson; Langerød, Anita & Naume, Bjørn [Show all 10 contributors for this article] (2010). Array comparative genomic hybridization (aCGH) differentiates the major intrinsic subgroups of breast carcinomas. European Journal of Cancer. ISSN 0959-8049. 8(5), p. 163–163.
Molloy, TJ; Bosma, AJ; Baumbusch, Lars Oliver; Borgen, Elin; Van't, Veer LJ & Naume, Bjørn (2009). A multi-marker QPCR panel for the detection of circulating tumor cells predicts survival in breast cancer patients. Cancer Research. ISSN 0008-5472. 69(2), p. 90S–91S.
Molloy, TJ; Bosma, A; Baumbusch, Lars Oliver; Borgen, Elin; Van't Veer, LJ & Naume, Bjørn (2009). A QPCR-Based Circulating Tumor Cell Detection Platform Predicts Survival in 776 Early Stage Breast Cancer Patients. Cancer Research. ISSN 0008-5472. 69(24), p. 647S–647S.
Molloy, TJ; Bosma, AJ; Baumbusch, Lars Oliver; Borgen, Elin; van't Veer, L & Naume, Bjørn (2009). A multi-marker QPCR panel for the detection of circulating tumor cells predicts survival in breast cancer patients.
Baumbusch, Lars Oliver; Geigl, J.B.; Riethdorf, S; Schneider, IJ; Mathiesen, RMR & Fjelldal, R [Show all 10 contributors for this article] (2009). Molecular signatures of disseminated tumour cells in metastatic breast cancer patients. Show summary
Background The critical step in breast cancer progression is the spreading of tumor cells to distant organs. Numerous studies have demonstrated that the presence of Disseminated Tumor Cells (DTC) in the bone marrow from patients with breast cancer is an independent prognostic factor for systemic relapse and breast cancer related death. Immunocytochemical methods for detection of DTC make it possible to identify single DTC in a population of normal cells. However, we still have limited knowledge about the biological and molecular characteristics of the DTC themselves. Complete genomic profiles and expression patterns have to be considered in order to understand tumor aggressiveness, clinical outcome and/or the disseminated tumor cell status. Material and methods Mononuclear cells from the bone marrow of metastatic breast cancer patients are transferred to slides, DTC are identified by immunocytochemical staining and isolated by micromanipulation. Samples are amplified by single cell whole genome amplification and the resulting amplified DNA is applied to high density whole genome Agilent CGH arrays. Results We tested and established the Single Cell array Comparative Genomic Hybridization (SCaCGH) technique in our laboratories in order to investigate the molecular signatures of DTC in metastatic breast cancer patients. DTC from the bone marrow of metastatic breast cancer with variable numbers of DTC per mononuclear cells were selected and analyzed. In a first pilot of about 10 patients we compare the genomic profiles of 1 – 3 DTC per patient in relation to each other and among different patients. Our preliminary results show concordance of the genetic profiles from different DTC within a patient and different copy number variations among the DTC from different patients. Conclusions Due to the implementation of a technique called the Single Cell array Comparative Genomic Hybridization (SCaCGH) we are finally able to characterize single cells using high density microarrays. This will provide us with information about the properties of DTC important for the understanding of the metastatic cascade, apart from the potential to better understand tumor heterogeneity in general. Acknowledgements This work has received research funding by the Communities Sixth Framework Programme as part of the international DISMAL collaboration for research into disseminated epithelial malignancies.

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Published Oct. 11, 2022 9:21 AM - Last modified June 30, 2023 1:24 PM